Journal: Journal of Advanced Research
Article Title: Targeting NGF but not VEGFR1 or BDNF signaling reduces endometriosis-associated pain in mice
doi: 10.1016/j.jare.2024.08.017
Figure Lengend Snippet: Levels of VEGFR1 ligands are increased in the peritoneal fluid of endometriosis patients and in mouse lesions. (A) experimental procedures for peritoneal fluid collection from endometriosis patients. (B) The levels of VEGFA, VEGFB, PlGF, and sVEGFR1 in peritoneal fluid samples determined by Ella®. Data are presented in box and whisker charts representing the levels of each mediator in pg/mL. (C) Calculated VEGFR1 occupancy as determined by previously published dissociation constant (K d ) and half maximal effective concentrations (EC 50 ) , , . Note that in published data, occupancy is approximately proportional to signalling . (D) Experimental procedures and timepoints for tissue collection in our mouse model. (E) 56 days after endometriosis induction, mouse lesions were collected for determination of VEGFA levels by ELISA. Results are presented as mean ± SEM of VEGFA levels, n = 10 (sham – uterine horn tissue), and n = 9 (endo) mice per group (*P<0.05 vs. sham by two-tailed Student’s t -test). (F) Profile of VEGFR1 expression in DRG nociceptors determined by immunohistochemistry 56 days after endometriosis induction.
Article Snippet: The concentration of NGF-β from patients’ peritoneal fluid and mouse endometriosis lesions was determined using a commercially available rat beta-NGF DuoSet ELISA kit (Cat# DY556, R&D systems, Minneapolis, MN, USA).
Techniques: Whisker Assay, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Expressing, Immunohistochemistry